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The "MITO - Medical Imaging TOolkit" project coagulates a number of activities aimed at defining and implementing an open-source, cross-platform software architecture for advanced Medical Imaging. MITO toolkit makes it possible to fetch radiological information and images stored in a PACS according to the standard format DICOM, then provides the final user with basic functionalities such as 2D-3D visualization (VR, SR, MIP), image segmentation and fusion, ROI. Moreover, MITO provides interaction techniques for manipulating 3D medical data in a virtual environment by 2 DOF input devices.