ImmunoRatio is a free web application for automated image analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67 immunostained tissue sections.
- segments diaminobenzidine-stained (DAB) and hematoxylin-stained nuclei regions from the user-submitted image,
- calculates the percentage of DAB-stained nuclear area over total nuclear area (=labeling index), and
- generates a pseudo-colored result image matching the area segmentation (see the example on right side).
ImmunoRatio is also available as a plugin version for the ImageJ image analysis software. For downloads and more details, please see the plugin webpage.